Drew Allar's three touchdown passes silenced the Mountaineers in Morgantown.
Penn State will be without tight ends Kahlil Dinkins and Jerry Cross in its season opener.
Penn State opens its season at noon on Saturday in Morgantown, West Virginia.
From "Come Get Her" to "Sunflower," Lee brought it all to LightsUP.
Penn State took an early lead before going down to only 10 men, allowing Pitt to secure a second half win.
After a rough start, the Nittany Lions won three straight sets.