Jess Mruzik headlined the group as a unanimous selection.
This marks the fifth time Gladieux has earned first-team All-Region recognition.
Penn State will play seven games at home and five away in 2025.
The freshman racked up 11.05 assists per set this season.
The third-year head coach led the Nittany Lions to a 29-2 regular season and conference title.
The Nittany Lions have lost eight straight games to Purdue dating back to 2020.