Penn State extended its losing streak to four games, its longest of the season.
Mustipher racked up 144 tackles during his five seasons at Penn State.
The Nittany Lions split the doubleheader thanks to two big home runs in game two.
Washington left Penn State with the 10th most receptions in program history.
Shorter appeared in 14 games for the Nittany Lions before transferring to Florida.
Twelve runs is the largest deficit the Nittany Lions have suffered all season.