After a sluggish start, Phia Gladieux scored a brace to win the Route 45 Rivalry.
The victory ended a three-game losing streak for the Nittany Lions.
The Nittany Lions rebounded from their previous outing, claiming victory from the Dukes in straight sets on Friday.
Aiden Fink is the only Penn State player on a preseason team.
Kailtyn MacBean recorded her 10th goal of the season in the win.
The Lady Lions begin Big Ten play on December 7 against Indiana.