The Nittany Lions have a 6-2 all-time record against the Bulls, but haven't faced Buffalo since December 2004.
Travis Luensmann had a season-high nine strikeouts en route to a dominant win on the road.
Penn State last won the Big Ten Tournament in 2019.
Penn State last won the Big Ten Tournament in 2015.
The Nittany Lions will face USC, UCLA, Washington, and Oregon for the first time as in-conference opponents.
Despite leading in the first set, Penn State couldn't keep pace in sets two and three and fell to the Anteaters.