Penn State fell to the Spartans in a top-ten match for only its second loss of the season.
The Nittany Lions move to 10-1 with the Sunday afternoon win.
After a sluggish start, Phia Gladieux scored a brace to win the Route 45 Rivalry.
The victory ended a three-game losing streak for the Nittany Lions.
The Nittany Lions rebounded from their previous outing, claiming victory from the Dukes in straight sets on Friday.
Aiden Fink is the only Penn State player on a preseason team.