Penn State took an early lead before going down to only 10 men, allowing Pitt to secure a second half win.
After a rough start, the Nittany Lions won three straight sets.
In the program's first game in its new home, the Nittany Lions kept it close against a talented Cavalier side.
Kaitlyn MacBean scored all three goals against the Mountaineers on Thursday.
Gladieux and the Nittany Lions start their season on Friday against Virginia.
The game was originally scheduled for 7:30 p.m.