The Nittany Lions lost their chance to win the Big Ten regular-season title following the Badgers' 82nd-minute goal.
The drop comes after the Nittany Lions' 20-12 defeat by the Buckeyes in Columbus.
Sophia Gladieux's brace lifted the Nittany Lions over the top-five-ranked Hawkeyes.
The Nittany Lions suffered their first loss of the season as their comeback against AIC fell short.
After his ninth loss to Ohio State, James Franklin refused to address concerns about Penn State's record against the Buckeyes.
Penn State's head coach went for it on 4th-and-4 in his own territory with seven minutes left in a close game.
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