The Nittany Lions defeated the top-ranked Buckeyes for the second time in three days.
With the victory, Penn State clinched its first winning record since 2016-17.
The Nittany Lions fell to 3-6 in Big Ten play.
Paquette scored the game-winning goal with four minutes left in the game.
Still without star goalie Josie Bothun, the Nittany Lions only allowed two goals on the weekend.
The Nittany Lions were on a four-game skid going into Friday's game.