Penn State spokesperson Lisa Powers said both fraternities can appeal the interim suspension.
Liam Butts and Brandon Hackenberg are both considered top 35 players in the nation, according to TopDrawerSoccer.
Over the past week, Penn State's total positivity rate for the semester jumped from 4.9% to 6.4%.
Levis entered the transfer portal just last week after spending his redshirt sophomore season primarily as Sean Clifford's backup.
The Nittany Lions' bye week will now be on October 16 and their date with Ohio State has moved up to October 30, among a few other changes.