No. 11 Alabama run ruled Penn State after five innings, then Virginia Tech used a strong fifth inning to power past the Nittany Lions, 3-1.
Penn State won the last four bouts to come back from a 13-9 deficit.
Despite Madison Carter's six-goal performance, the Nittany Lions narrowly fell to the Tigers in Saturday's season opener at Johnny Unitas Stadium.
After trailing by one to start the second half, Penn State scored seven unanswered goals to secure a 15-8 win over the Hobart Statesmen in Holuba Hall on Saturday.
The fifth-year head of the Nittany Lions program previously coached the United States national team for the Junior and Senior World Championships in 1989-1998, the Pan American Games in 1991, and the Olympic Teams in 1992 and 1996.
Left back Anthony Harding and forward Jeremy Rafanello officially signed their letters of intent Thursday and are set to join Jeff Cook's program next season.