The Nittany Lions leaped out to a 3-0 lead and were able to hang on through pressure for the 3-2 win.
Penn State was able to make a statement against its toughest opponent of the season thus far.
The Nittany Lions drop one spot from No. 6 last week.
Jalen Pickett finished with a double-double and was named to the All-Tournament Team for the Charleston Classic.
Goals from Payton Linnehan and Amelia White weren't enough to outlast a powerful Virginia offense.
The Nittany Lions were shut out 3-0 in game one before dropping game two 2-1 in overtime.