Penn State scored four unanswered goals in the second period, but Wisconsin responded with five straight tallies of their own to hand Penn State its third regulation loss of the season.
The Nittany Lions picked up their 100th win in NCAA tournament play Saturday night at Rec Hall following a sweep of the Orange.
Penn State dropped its conference opener on the road, despite leading at halftime.
Alex Limoges' shootout winner gave the Nittany Lions the extra point in their first game of a two-game set with the Badgers.
The Nittany Lions beat Bucknell 42-3 in front of a sold-out Sojka Pavilion with more than 4,200 fans in attendance.
The Nittany Lions will host Syracuse Saturday at 7 p.m. at Rec Hall in the second round of the NCAA tournament.