Brookins was with the Cyclones for one season, but preserved his redshirt and did not see any game action.
Ebel becomes the third Iowa State linebacker to transfer to Penn State.
Buhr logged over 1,000 snaps at left guard in his time with the Cyclones.
After redshirting in 2025, Henderson has four years of eligibility remaining.
Jennings follows James Franklin and several others to Blacksburg.
Brezina totaled 51 tackles, three tackles for loss, one forced fumble, and one fumble recovery across his Cyclone career.