Both goalies stood on their heads, but Dylan Foulds' clutch performance in the final seconds was enough for a key victory.
The Nittany Lions took game one thanks to a strong defensive performance, but fell in overtime to the Buckeyes Saturday afternoon.
Rutgers' offense was simply too much for Penn State as it jumped out to a big lead to start and never looked back.
Gladieux was also named the conference's Freshman of the Week after scoring five goals in last weekend's series sweep over Michigan State.
O'Keefe is now only 10 goals shy of being the all-time leader in NCAA history.
Freshman Sophia Gladieux was a star in the first game of the weekend, clearing the way for a series sweep of the Spartans.