Penn State plays No. 5 seed USC at 3 p.m. on Thursday, May 22 to finish pool play.
Williams and Rowling are the fifth and sixth players to enter the transfer portal.
Missy Doherty stepped down from the helm of the Nittany Lions women's lacrosse program on May 1 after 15 years leading the blue and white.
Penn State opens as 43.5-point favorites in season opener after preseason hype.
"When you get to this point, we talk to our guys a lot about bond and belief."
Paxton Kling, Jack Porter, and Cole Wagner all earned Big Ten accolades.
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